Compensating Light Intensity Attenuation in Confocal Scanning Laser Microscopy by Histogram Modeling Methods
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چکیده
منابع مشابه
Robust incremental compensation of the light attenuation with depth in 3D fluorescence microscopy.
Summary Fluorescent signal intensities from confocal laser scanning microscopes (CLSM) suffer from several distortions inherent to the method. Namely, layers which lie deeper within the specimen are relatively dark due to absorption and scattering of both excitation and fluorescent light, photobleaching and/or other factors. Because of these effects, a quantitative analysis of images is not alw...
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Background To report normal variations of the limbal structures using in vivo laser scanning confocal microscopy. Methods: This was a retrospective study of fourteen eyes from 11 healthy individuals. Confocal imaging of cornea and limbus was performed using Heidelberg Retina Tomograph III Rostock Corneal Module. Results: The typical structure of the palisades of Vogt (POV) was detected ...
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We present a transmission-mode confocal laser scanning microscope system based on the use of second-harmonic generation (SHG) for signal detection. Our method exploits the quadratic intensity dependence of SHG to preferentially reveal unscattered signal light and reject out-of-focus scattered background. The SHG crystal acts as a virtual pinhole that remains self-aligned without the need for de...
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Fading is one of the major obstacles to reliable observation in fluorescence microscopy. Using a confocal laser scanning microscope (CLSM) coupled to a computer, we quantitatively measured fading of fluorescence to formulate an equation, evaluated the anti-fading ability of several anti-fading media, and restored the faded images to the original level according to this equation. NIH 3T3 cells w...
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Intensities of fluorescent images captured by a confocal laser scanning microscope (CLSM) from deep layers of a specimen are often darker than images from the top most layers due to absorption and scattering of both excitation and fluorescent light. These effects cause problems in subsequent analysis of biological objects. Methods [e.g. 1, 2] used for solution of this problem are generally base...
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